Studies with Deuterium Steroids 



37 



containing less pure astiocholanolone and androsterone and 



mixtures of these two were combined, and known quantities 



of non-isotopic androsterone and aetiocholanolone were added 



as carriers. A second chromatographing, followed by careful 



purification of the products, was performed and analytically 



pure samples of androsterone and aetiocholanolone were 



obtained. Knowing the amount of carrier added and the 



deuterium content of the excreted androsterone and the 



excreted astiocholanolone, it was readily calculated that 4-2 



mg. of androsterone and 12-7 mg. of aetiocholanolone had 



been combined. Adding these values to the weight of pure 



androsterone removed from the first chromatogram (9-0 mg.) 



and to the pure aetiocholanolone similarly removed (22-0 



mg.) afforded the total excretion of these two compounds. 



Since the total amount and the dilution were known, both 



endogenous production and the quantity obtained from the 



administered hormone were calculated. These results are 



shown in Table I. 



Table I 



Urinary Excretion during 24 Hours after 100 mg. Testosterone 



Intramuscularly in Man 



17KS = 70-5mg. a = 67-8 mg. j3 = 0-6 mg. 



The values for the quantitatively minor ketonic metabolites 

 were obtained by isotopic dilution alone since the amounts 

 were too sm^all to permit direct isolation. In these instances, 



