Studies on Mammalian Red Cells 



75 



differences between animals, but again the ratios of activities 

 may be expected to be significant. 



The results (Figs. 3 and 4, Table I) show that at least in 

 experiments in which samples were taken 24 hours or earlier 

 after the administration of the labelled glycine, the ratios of 

 the molar activities of the porphyrin and of the globin glycine 



O 10 



Specific Activity -Time Curves 





10 



20 



30 

 TIME (DAYS) 



40 



50 



Fig. 3. Radioactivities of glycine 



/ counts X molecular weight 



1000 



) 



/counts X molecular weight \ ,. . 

 and protop orphynn I — — I obtamed irom 



\ JL vvl v/ /\ O y 



a rabbit which had received 57 fic NHg-^^CHg-COOH and 100 



IXC CH3.14COOH. 



were fairly close to eight. The individual values varied 

 between seven and nine, but the average was 7-9. However, 

 during the first 24 hours this ratio was between 5*2 and 6-8. 

 Such low values might suggest that the globin is formed 

 somewhat later in the erythropoiesis than the porphyrin. 

 Such an assumption would not contradict the findings of 

 Thorell (1947) who established by spectrophotometric methods 



