104 



HOLLAENDER, StAPLETON AND BURNETT 



t rat ions (see Fig. 7). Moreover, sodium hydrosulphite affords 

 some protection in nitrogen-saturated suspensions. This 

 compound appears to protect by depletion of oxygen from 

 the suspensions, possibly by removing bound oxygen from 



wo- 



400- 



o 



3O0- 



o 



> 



w 

 $200- 



3 



100- 



ETHANOL 

 2' 



-| 1 T- 



0,2 03 04 



MOLAR CONCENTRATION 



—r- 



06 



Fig. 6. Relative survival of E. coli in different concentrations 

 of ethyl alcohol at 2»C. and 37«C. Stapleton, Billen and 



Hollaender (in preparation). 



the bacterial cells (Morse, Burnett, Burke, and Hollaender, in 

 preparation). 



Some of the carboxylic acids which are normal metabolic 

 intermediates or end products have also proved to afford 

 excellent protection to bacterial cells, but again, under rather 

 restricted conditions. A short incubation period for the cells 

 in the presence of the acids produces a striking increase in 



i 



