STAINING, PRACTICAL AND THEORETICAL 



SOLOCHROME CYANIN R 



A simple polychrome stain for neurological and general 



tissues 



(E. Gurr & M. A. MacConaill, 1959) 



Solution required: 



Solochrome cyanin R (Michrome 



No. 750) 0-5 gm. 



Glacial acetic acid . . . . 8 ml. 



Water . . . . . . . . 92 ml. 



Technique [for neurological tissues) : 



1. Fix pieces of brain or spinal cord in 10% formaldehyde. 

 Note: Do not use formaldehyde that has been neutralized with 



metallic compounds. 



2. Demyelinate by treatment with successive baths of 96% 

 alcohol, anhydrous cellosolve and anhydrous pyridine. 



3. Embed in paraffin wax and cut sections at 8ju,. 



4. Fix sections to slides and carry through xylol to water in the 

 usual way. 



5. Immerse in the staining solution for five to eight minutes. 



6. Wash in running water for five minutes. 



7. Dehydrate through the usual graded alcohols. 



8. Clear in xylol. 



9. Mount in D.P.X. or Clearmount of neutral Canada balsam 

 in xylol. 



Results: 



Erythrophile* (basic) tissue elements: pink to red. Acidic 

 tissue elements are stained in shades of blue. 



Notes: 



(a) The picture of the tissue elements is not nearly so clearly 

 marked as with the Falg or the Faviol techniques (pages 

 198-200, 203-6). 



(b) For further information, reference should be made to 

 pages 9-10 of this book. 



References: 



* MacConaill, M. A. (1949, 1951). 



Gurr, E. & MacConaill, M. A. (1959)- 



422 



