SECTION TWO 



6. Transfer the sections to distilled water for three to five 

 minutes. 



7. Counterstain with carmalum for about three minutes. 



8. Wash with distilled water. 



9. Mount in glycerine jelly or Aquamount. 



Results: 



Lipid substances or particles are stained blue-black to black. 

 Nuclei: red. 



Reference: Chiffelle, T. L. & Putt, F. A. (195 O- 



SUDAN BLACK 

 For myelinated peripheral nerves 



Solutions required: 



A. McManiis fluid 



Cobalt sulphate or nitrate 

 Calcium chloride, 10% . . 

 Distilled water 

 Formalin (40% formaldehyde) 



B. Formic acid, 50% 

 Sodium citrate, 20% 



Note: Solution B is only required when decalcifi- 

 cation is necessary. 



C. Gelatine, 20% 



D. Gelatine, 5% 



E. Gelatine, 10% 



F. Gelatine, 1% 



G. Sudan black, saturated in 70% 



alcohol 



Note: Solutions of Sudan black in alcohol deterior- 

 ate after a few weeks and I would, therefore, suggest 

 that ethylene or propylene glycol be used instead as 

 either of these two glycols give stable solutions of the 

 dye {see page 428). 



429 



