SECTION TWO 



Stained, while nodes of Ranvier and the varicose character ot the 

 myelin sheath are clear. The author (Stilwell) claims that even 

 some details of intrafusal endings and somatic motor terminations 

 can be seen in sections over ^ojx in thickness. 



Notes: 



(a) Myelin nerves have been demonstrated by Stilwell in this 

 technique, in sections of bone, periosteum, ligament, skeletal 

 muscle, the fibrous capsule of the diarthrodial joints and in fascia 

 and other connective tissues, and readers are referred to the 

 original paper for more detailed information and photomicro- 

 graphs. 



(b) It is claimed that as a method of studying peripheral nerves, 

 practically all of which have myelinated motor or sensory fibres, 

 this technique is of advantage because of its simplicity, and 

 because it demonstrates the finest detail in myelin whilst obviating 

 a background stain in tissues relatively free of adipose tissue. 



Reference: Stilwell, D. L. (1957). 



SUDAN BLACK 

 J. R. BAKER'S PYRIDIN EXTRACTION METHOD 



For confirmation of lipids 



Techtiique: 



I . Fix pieces of tissue in reagent A for twenty-four hours. 

 7. Wash in 70% alcohol to remove excess picric acid. 

 3. Cut frozen sections and immerse some of them in pyridine 

 for thirty minutes at 22° C. 



431 



