STAINING, PRACTICAL AND THEORETICAL 



SUDAN 2-HAEMATOXYLIN 

 For degenerating and intact myelin and fat 



Solutions required: 



A. Haematoxylin i% in absolute 

 alcohol, at least one to five days 

 old . . . . . . . . 50 ml. 



4% iron alum aqueous . . • . 50 ml. 



This solution should be prepared immediately 



before use. 



Technique: 



1 . Formalin-fixed frozen sections are stained forty minutes with 

 Solution A at 56° C. in a covered dish in an oven. 



2. Rinse in water and diff"erentiate for one hour with Solution B. 



3. Rinse in distilled water and immerse for ten minutes in 

 Solution C. 



4. Stain ten to twenty minutes in Solution D ; then float out in 

 water. 



5. Mount in Apathy's gum syrup or in Aquamount. 



Results: 



Degenerating myelin and fat: black; normal myelin, blue-black ; 

 nerve cells, grey; nuclei, deeper grey; red corpuscles, yellow to 

 black ; fats, orange yellow. 



Reference: Lillie, R.*D.[(i948), p. 161. 



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