SECTION TWO 



Results: 



Nuclei of amoebae are stained a rich brown colour, while the 

 nuclei of all other cells are stained blue. 



Reference: Mallory, F. B. (1938), pp. 2, 297. 



THIONIN (Ehrlich) 

 For nerve cells and fibre tracts 



Solution required: 

 Staiti and fixative combined: 

 Thionin 0-3% in 10% formalin. 



Technique : 



1. Tissues are fixed and stained simultaneously by immersing 

 in the above solution for a few days to three months. 



2. Wash well in running water. 



3. Dehydrate in ascending grades of alcohol in the usual man- 

 ner. 



4. Clear; embed in paraffin wax or in Celloidin. 



5. Fix sections to slides and mount in Cristalite or D.P.X. 



Results: 



Cell bodies are stained blue, while the fibre tracts are red. 

 Reference: Chang, M. (1936). 



THIONIN (Ehrlich) 

 For demonstrating malignant cells in biopsy material 



Solution required: 



Thionin 1% aqueous. 



Technique: 



I. Stain frozen sections for ten to sixty seconds in the thionin 

 solution. 



439 



