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SECTION TWO 



9. Clear in xylol and mount in D.P.X. or Clearmount or 

 Emexel. 

 Note: Instead of solution A, the following may be used: 



Thionin, 1% aqueous . . 0-5 ml. 



Distilled water . . . . 10 ml. 



References: 



Bensley, S. H. (1934). 

 Jones, Ruth M. (1950). 



TOLUIDINE BLUE 

 For Nissl granules 



Solution required: 

 Toluidine blue, 1% aqueous 



Technique: 



1. Human neurological material is fixed in formol saline for at 

 least three days. 



2. Dehydrate in alcohol as usual and clear in chloroform. 

 Alternatively dehydrate and clear in pyridine (as page 502). 



3. Stain in the toluidine blue solution for one and a half hours. 



4. Rinse very rapidly in tap water. 



5. Drain off excess water (but do not allow the sections to dry) ; 

 then blot gently. 



6. Differentiate and dehydrate rapidly with absolute alcohol. 



7. Clear in xylol. 



8. Mount in D.P.X. 



Results: 



Nissl granules: bluish purple. 



Notes: 



(a) The author states that it is not necessary to use Gothard's 

 differentiator, which in any case was found to be too strong 

 even when diluted to half strength with absolute alcohol. 



(b) For more detailed information and photomicrograph, 

 readers should consult the original paper. 



Reference: Irugalbandara, Z. E. (i960). 



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