STAINING, PRACTICAL AND THEORETICAL 



TOLUIDINE BLUE 

 For mucus 



Solution required: 

 Toluidine Blue 1-5% aqueous. 



Technique: 



1. Formalin-fixed material is embedded in paraffin wax in the 

 usual manner. 



2. Bring sections down to distilled water then stain for one or 

 two minutes in the toluidine blue solution. 



3. Wash with distilled water; drain well, then plunge the slide 

 into two changes of 95% alcohol. 



4. Dehydrate by immersing in two changes of acetone; then 

 clear in xylol and mount. 



Results: 



Mucus, reddish violet; nuclei, blue; erythrocytes, yellow to 

 greenish yellow. 



TOLUIDINE BLUE 



For the identification and counting of basophil leucocytes 



(After A. W. A. Boseila, 1959) 



Solutions required: 



A. Toluidine blue . . . . . . i gm. 



Alcohol, 60% . . . . . . 100 ml. 



B. Toluidine blue . . . . . . i gm. 



Alcohol, 40% 100 ml. 



C. Sorensen phosphate buffer solu- 



tion pH 77 



Note: The most convenient way of preparing this 

 is merely to dissolve a Michrome buifer tablet, pH 

 7-7, in 100 ml. of distilled water 



D. Saponin . . . . . . . . 2-8 gm. 



Alcohol, 50% 100 ml. 



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