E. 



For blood and bone marrow sfnears 



1. Air-dried smears are fixed in absolute methyl alcohol for 

 ten to twenty minutes. 



Note: In the case of human blood, thick smears are recom- 

 mended on account of the scarcity of basophil leucocytes. 



2. Stain in solution A for thirty seconds to one minute. 



3. Immerse for thirty seconds in 50% alcohol. 



4. Drain off excess liquid and blot dry. 



Technique II 



For sections 



1 . Fix small pieces of tissue in absolute methyl alcohol for ten 

 to twenty-four hours. 



2. Clear in xylol. 



3. Embed in paraffin wax. 



4. Fix sections to slides and remove paraffin wax with xylol. 



5. Wash in absolute alcohol (methyl or ethyl). 



6. Wash in 90% alcohol (methyl or ethyl). 



7. Wash in 70% alcohol. 



8. Immerse in solution B (toluidine blue in 40% alcohol) for 

 twenty minutes. 



9. Wash in 70% alcohol. 



10. Wash in 90% alcohol. 



11. Dehydrate in absolute alcohol. 



12. Clear in xylol. 



13. Mount in Michrome mountant or proceed as follows: 



443 



