SECTION TWO 



5. Immerse the preparation in 3% phosphotungstic acid for 

 ten to fifteen minutes. 



6. Wash gently under the tap for one minute. 



7. Stain in solution G for five to twenty minutes. 



Note: The time is not critical although results will be slightly 

 diflFerent. 



8. Rinse in 2% acetic acid. 



9. Dehydrate, clear and mount. 



Results: 



Nuclei: blue. Cytoplasm, muscle fibres, red cells, etc., in shades 

 of red. Connective tissue : green or blue. 



Note: Professor Gomori used Woodstain scarlet, which was 

 not available under that name in Britain. The British equivalent 

 at that time being Neoponceau. It is felt that this synonym is 

 more suitable in this case, as the name Woodstain scarlet, indexed 

 in literature on general biology, might suggest a botanical stain 

 for woody tissues. 



References : 



Gomori, G. (1950), Personal communication. 

 Gurr, E. (1956). 



TRICHROME STAIN (Masson), Modified 



For epithelium, pituitary and thyroid glands, nerve (normal 



and tumour), etc. 



Solutions required: 



A. Regaud's haematoxylin. 



B. Picric acid saturated in 95% alcohol 20 ml. 

 Alcohol 95% ID ml. 



C . Viol amine- acid fuchsin : 



Violamine, R . . . . . . 0-7 gm. 



Acid fuchsin 0-3 gm. 



Glacial acetic acid . . . . i ml. 



Distilled water 100 ml. 



D . A cetic fast green 



Fast green, F C F . . • • 3 gni- 



Glacial acetic acid . . . . 2 ml. 



Distilled water . . . . . . 100 ml. 



451 



