SECTION TWO 



10. Pour off excess acid ; rinse well in distilled water; drain and 

 blot carefully. 



11. Rinse well with acetone. 



12. Rinse with two washings of Solution F. 



13. Clear in xylol and mount. 



Results: 



Muscle and cytoplasm: red. Connective tissue and basement 

 membranes : green to dark blue. 



Reference: Lillie, R. D. (i945)- 



WRIGHT'S STAIN 



For general differentiation of blood corpuscles; for 

 malarial parasites; trypanosomes, etc. in sections. 



This stain is extensively used in America instead of Leishman 

 stain which appears to be preferred by many British workers. 



Solutions required: 

 Formol- saline, neutral, hujfered: 



A. Formalin (40% formaldehyde) . . 100 ml. 

 Sodium chloride, A.R. . . . . 8-5 gm. 



Distilled water . . . . . . i litre 



Acid sodium phosphate, mono- 

 hydrate . . . . . . • • 4 gn^- 



Anhydrous disodium phosphate 6-5 gm. 



B. Wright's stain. 



C. Acetic acid, o-o8% aqueous. 



Technique: 



1. Fix pieces of tissue in Solution A for sixteen to forty-eight 

 hours. 



2. Dehydrate in the usual ascending grades of alcohol; clear 

 and embed in paraffin wax. 



3. Fix sections, not exceeding 5/1 in thickness to slides; remove 

 wax with xylol ; pass through descending grades of alcohol down 

 to neutral distilled water. 



465 



