SECTION TWO 



for five minutes with pure methyl alcohol ; then blotted and dried 

 at room temperature. 



1. Place I ml. of the stain on a dried blood film and leave it to 

 act for one minute; then add 2 ml. distilled water and rock the 

 slide gently to mix. 



2. Allow this diluted stain to act for three to five minutes ; then 

 pour off and wash with distilled water until the thin portion of the 

 films appears pink to the naked eye. 



3. Pour off excess stain; blot and dry at room temperature. 



Results: 



Erythrocytes: yellowish red. Polymorphonuclears: dark purple 

 nuclei, reddish violet granules, pale pink cytoplasm. Eosinophiles : 

 blue nuclei, red to orange-red granules, blue cytoplasm. Baso- 

 philes : purple to dark blue nuclei, dark purple to black granules. 

 Lymphocytes : dark purple nuclei, sky-blue cytoplasm. Platelets : 

 violet to purple granules. Malarial parasites and Leishmania: 

 chromatin, red ; cytoplasm, blue. Trypanosomes : chromatin, red. 



Note: The timing of the staining either before or after dilution 

 may be altered to suit individual requirements. 



Staining effects similar to Giemsa are obtained by staining for 

 ten minutes in Wright's stain diluted with four times its volume of 

 distilled water buffered to pH 6-5. 



Reference: Wright, J. H. (1902). 



467 



