STAINING, PRACTICAL AND THEORETICAL 



Carnoy's Fluid 



Recommended for : 



Glycogen ; for animal tissues in general, and for plant cytology. 



Recipe: 



Absolute alcohol . . . . . . 60 ml. 



Chloroform . . . . . . • • 30 ml. 



Glacial acetic acid . . . . . . 10 ml. 



Technique: 



Fix for one and a half to two hours; then transfer to two 

 changes of absolute alcohol before clearing and embedding. 



Remarks: 



A rapid fixative with great penetrating power. 



Carnoy - Le Brun Fluid 



Recommended for : 



Insects and ticks, and as a rapid and penetrating fixative for 

 plant tissues. 



Recipe: 



Absolute alcohol . . . . • • 30 ml. 



Glacial acetic acid . . . . • • 30 ml. 



Chloroform . . . . . . • • 30 ml. 



Mercuric chloride . . to saturation (about 10 gm.) 



Technique: 

 Fix from half to one minute ; then wash in 95% alcohol. 



Champy's Fluid 



Recommended for : 



Plant and animal tissues in general; mitochondria and other 

 cytological detail. 



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