(Photograph by courtesy of Sandoz Ltd., Basle, Sicitzerland) 



Normal and Malignant Cell Elements as Observed tvith the Cytodiagnostic Acridine Orange 

 Fluorescence Microscope Method {by Bertalanffy) 



Top left. — Normal vaginal aspirate. In the upper portion of the picture are various 

 types of squamous cells from the vaginal epithelium. The superficial cells have green 

 cytoplasm and small green, pyknotic nuclei. The cytoplasm of the intermediate, para- 

 basal and basal cells fluoresces brown to reddish brown; the nuclei are larger and 

 greenish yellow. In the lower portion is a group of endocervical cells with yellow 

 fluorescent nuclei that partially obscure the scanty reddish brown cytoplasm. Scattered 

 between the squamous cells are green fluorescent, lobulated nuclei of polymorph 

 granulocvtes. +38, x 150. Top right. — Cervical aspirate from a case with squamous 

 cell carcinoma of the cervix. The malignant cells show flaming red cytoplasmic fluo- 

 rescence and yellowish nuclei. '+ 71, x 300. Centre left. — Normal bronchial secretions 

 showing several respiratory epithelial cells with reddish brown cytoplasm and greenish 

 yellow nuclei. These cells are surrounded by green nuclei of neutrophil granulocytes. 

 'S 56, X 250. Centre right. — Bronchial secretions from a case with adenocarcinoma 

 of the lung. The malignant cells have bright red cytoplasmic fluorescence and yellow 

 hvperchromatic nuclei. V57, x 360. Bottom left. — Pleural effusion showing a group 

 of malignant cells of pulmonary origin. The malignant cells have bright orange fluo- 

 rescent cvtoplasm. o 60, x 360. Bottom right. — Ascitic effusion with a group of 

 adenocarcinoma cells of ovarian origin. The cytoplasm of the malignant cells is flaming 

 red. Note the large red fluorescent, RNA-containing nucleoli. 'I 78, x 450. 



