SECTION THREE 



Zenker's Fluid 



Recommended for : 



Perfect histological detail in animal tissues in general. 



Recipe: 



Potassium dichromate 2-5% aqueous 100 ml. 

 Mercuric chloride . . . . • • 5 gm. 



Glacial acetic acid . . . . • • 5 ml. 



Technique: 



Immerse slices of tissue in the fluid for six to twenty-four hours 

 according to the nature of the material and the thickness of the 

 slices. Wash in running water for twelve to twenty-four hours; 

 then transfer to 80% alcohol. 



Remarks: 



Unsuitable for Mitochondria. Suitable for Mallory's connec- 

 tive tissue stain; for demonstration of Muscle, Fibrin, Haemo- 

 fuscin, Purkinje cells, etc. 



485 



