STAINING, PRACTICAL AND THEORETICAL 



DEHYDRATION, CLEARING, EMBEDDING, 

 SECTIONING, ETC. 



CELLOIDIN METHOD FOR EMBEDDING TISSUES 



For preserving the relations of cell layers of different con- 

 sistency, as are contained in the eye; for large objects; 

 for pieces of central nervous system ; and for hard tissues 



such as decalcified bone. 



Solutions required: 



A. Celloidin 8% 



Celloidin flakes . . . . • • 25 gm. damped 



with absolute alcohol* 

 Absolute alcohol . . . . . . 150 ml. 



Ether . . . . . . . . 163 ml. 



*Note: This may be obtained in 25 -gm. bottles, 

 ready damped with absolute alcohol. 



Pour the alcohol and ether into a clean, absolutely 

 dry, wide-mouth bottle of about 32 ounce capacity, 

 and mix by inverting the bottle several times, re- 

 leasing the stopper at intervals, to release the pressure 

 of ether vapour; then add the Celloidin flakes and 

 invert the bottle as before. Leave for about 12 hours 

 to dissolve ; inverting or shaking the bottle gently at 

 intervals. 



B. Celloidin 4% 



Celloidin solution 8% . . . . 2 volumes 



Absolute alcohol , . . . . . i volume 



Ether . . . . . . . . i volume 



Mix as described above, in a large-stoppered wide- 

 mouth bottle. 



C. Celloidin 2% 



Celloidin 4% . . . . . . 2 volumes 



, Absolute alcohol , , 



Ether 



D. Cedarwood oil, for clearing 

 Chloroform 



486 



I volume 

 I volume 



I volume 

 I volume 



