STAINING, PRACTICAL AND THEORETICAL 



the temperature at which the mohen paraffin wax sets, or the 

 temperature at which the sohd wax just begins to melt. Therefore 

 it may happen that such a wax with a stated melting point of, say, 

 58° C. will not be completely melted until the temperature is 

 taken up several degrees beyond this point. 



POLYESTER WAX 



A ribboning embedding medium 



(H. F. Steedman, 1957) 



Reagents required: 



A. Polyester wax 

 Polyethylene glycol (400) di- 



stearate 990 gm. 



Cetyl alcohol 10 gm. 



Warm gently in a pan over an electric hotplate 

 till melted; then stir well and put the preparation 

 aside to cool in the atmosphere. 



B. Distilled water i litre 



Nipa ester No. 82 1 21* .. .. I'Sgm. 



Boil the water first, then while the water is still 

 boiling add the Nipa ester and stir in until dissolved. 



C. Solution B (cold) . . . . 200 ml. 

 Amylopectin . . . . . . 2 gm. 



D. Amylopectin adhesive 



Solution B 800 ml. 



Solution C 200 ml. 



First raise the temperature of solution B to boiling, 

 then add slowly, with stirring, solution C. On 

 cooling this reagent is ready for use. 



Note: Solution D should not be filtered, and it 

 should always be shaken before use. 



Technique: 



I. Take pieces of fixed material into 96% alcohol through to 

 absolute alcohol and xylol. Alternatively the material can be 

 taken from 96% alcohol into cellosolve, if desired. 



* Made by Nipa Laboratories Ltd., Pontypridd, Glam., S. Wales. 



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