SECTION THREE 



Add solution A to solution B ; shake thoroughly ; then filter and 

 add about o-i gm. camphor as a preservative. 



Keep in a well-closed bottle to prevent evaporation. 



Reference: Wotton, R. M. & Zwemer, R. L. (i935)- 



LACTOPHENOL 



Refractive index i -460 



This is a non-drying medium used for making fluid mounts of 

 objects that are difficult or impossible to mount in Canada balsam 

 in xylol or other solidifying mountants. Lactophenol is used for 

 small whole mounts, such as insects, mites, mosses, algae, micro- 

 fungi, and small coelenterates. It is in universal use by mycolo- 

 gists. 



Dade (i960) states that a particular virtue of the lactophenol 

 method is that, in its simplest form, manipulation is completed in 

 a single operation. It is, therefore, especially suited to fragile 

 objects that cannot withstand repeated transfers from one fluid 

 to another without disintegration. Various stains can be incor- 

 porated in lactophenol, which then acts as a combined fixative, 

 staining agent and mountant. The lactic acid, which is an ingre- 

 dient of lactophenol, prevents delicate material from collapsing, 

 and it will, in fact, cause dried, shrunken cells to expand and to 

 appear to regain the natural forms they possessed when living. 



For further information, reference should be made to the 

 original paper by Dade (i960). 



REYNE'S MOUNTANT 

 For the preservation and mounting of small insects 



Chloral hydrate 50 gm. 



Water . . . . . . . . • • 50 ml. 



Glycerin . . . . . . . . 12-5 ml. 



Gum arable, white, powdered . . 30 gm. 



519 



