STAINING, PRACTICAL AND THEORETICAL 



Dissolve the chloral hydrate in the water, then sprinkle the 

 powdered gum on the surface of the solution and stir occasionally. 

 Leave overnight, then when all the gum has gone into solution, 

 stir in the glycerin and allow the preparation to stand overnight, 

 to get rid of air bubbles, before it is used. 



Note: 



It is claimed that preparations mounted in Reyne's fluid are 

 exceptionally clear and require no sealing or heating. This 

 medium, which is said to harden within a few days, has been 

 used for mounting dissected crustaceans such as copepodes. 



Reference: Reyne, A. (1950). 



UVAK 



(Edward Gurr) 



An aqueous mountant for fluorescence microscopy. Uvak is 

 moderately quick-drying and it sets hard without crystallizing. 

 The mountant is of particular use for making permanent pre- 

 parations of specimens that have been stained with acridine 

 orange and other fluorescence dyes which are removed from 

 tissues by dehydrating and clearing fluids. 



520 



