STAINING, PRACTICAL AND THEORETICAL 



about ten minutes stir until the gelatin is completely dissolved. 

 Add and stir in 80 ml. of absolute alcohol. 



Method of use: 



1. Immerse frozen sections, 15-50/x in thickness in the ad- 

 hesive for five minutes or longer. 



Note: The depth of the liquid should be at least 2-5 cm. 



2. Transfer sections to slides by means of a fine brush. 



3. Allow excess fluid on the slides to evaporate. 



4. Blot sections on slides with filter paper. 



Note: It may be necessary to damp the filter paper with 95% 

 alcohol. 



5. Immerse the slides, with the sections on them, in 95% 

 alcohol. 



Result: 



The remaining gelatin on the slides will congeal, holding the 

 sections firmly attached to the slides. If necessary, the sections 

 can be subsequently coated with celloidin solution (0-5% celloidin 

 in equal volumes of absolute alcohol and ether). 



Notes: 



The method was developed to overcome the difficulty of teasing 

 frozen sections, particularly large ones, on to slides without their 

 becoming dry and perhaps damaged in the process. 



The method is of particular value for large frozen sections of 

 brain tissue. 



Reference: Albrecht, M. A. (1954). 



GLYCERIN - ALCOHOL 



For softening brittle or crumbly material already 

 embedded in paraffin wax 



Solution required: 

 Absolute alcohol . . . . • • 54 ml. 



528 



