SECTION THREE 



Water . . 

 Glycerine 



36 ml. 

 10 ml. 



Technique: 



1. Cut the block at one side so that the surface of the tissue 

 is exposed. 



2. Soak the block in glycerin-alcohol for several hours or over- 

 night or longer if necessary. 



Results: 



The glycerin-alcohol penetrates and softens the tissue, despite 

 the presence of the paraffin wax, and makes section cutting 

 easier. 



Notes: 



Soaking in water has a similar effect, but glycerin-alcohol 

 penetrates more quickly and makes the tissue softer : also macera- 

 tion is avoided and glycogen and water-soluble proteins are not 

 extracted. X^^^^/!^ 



References : 



Baker, J. R. (1941). 1^1^ ^i^^^i^ 



Carleton, H. M. & Leach, E. H. (1937). Y'^^A I IQR 



HAUPT'S ADHESIVE 



Gelatine powder 



Water . . 



Phenol, 5% aqueous . . 



Glycerine 



Warm 50 ml. of the water to 50-60° C. in a beaker. Sprinkle 

 the gelatine powder on the surface of the water. Leave for about 

 fifteen to thirty minutes for the gelatine to dissolve, then stir 

 well and add the glycerine. With the remaining 10 ml. of water 

 rinse out the measuring cylinder that has been used for the 

 glycerine. Add the rinsings to gelatine-glycerine mixture. Add 

 the phenol solution ; stir well ; then filter. 



529 



