STAINING, PRACTICAL AND THEORETICAL 

 RECIPES 



SUNDRY FIXATIVES 



ALTMANN'S FLUID 



Osmic acid 2 % . . . . . . . . i volume 



Potass, dichromate 5 % . . . . . . i volume 



5-AMINOACRIDINE HYDROCHLORIDE FIXATIVE 



For acid mucopolysaccharides 



(After G. Williams & D. S. Jackson, 1956) 



5-Aminoacridine hydrochloride . . . . 2 gm. 



Absolute alcohol . . . . . . . . 50 ml. 



Distilled water . . . . . . • • 50 ml. 



Thin slices of tissue are fixed for forty-eight hours; then washed 

 thoroughly with 50% alcohol to remove excess 5-aminoacridine 

 hydrochloride before dehydration and embedding in paraffin wax. 



Note: The authors tested this fixative on thin slices of human and 

 guinea pig tissues, comprising costal cartilage, aorta, stomach and 

 kidney. 



AOYAMA'S FIXATIVE 



Cadmium chloride 1% . . . . . . 200 ml. 



Formalin (40% formaldehyde) . . . . 80 ml. 



Distilled water . . . . . . . . 220 ml. 



APATHY'S FIXATIVE 



Mercuric chloride 5% aqueous . . . . 200 ml. 



Acetic acid 10% aqueous . . . . . . 25 ml. 



Distilled water . . . . . . • • 75 nil. 



Absolute alcohol . . . . . . . . 200 ml. 



BENDA'S FLUID 



Osmic acid 2 % . . . . . . . . 20 ml. 



Chromic acid i % . . . . • • 75 nil. 



Glacial acetic acid . . . . . . . . i ml. 



Note: The solution should be freshly prepared. 



532 



