STAINING, PRACTICAL AND THEORETICAL 



CRETIN'S FIXATIVE 



Picric acid, saturated, aqueous 

 Mercuric chloride . . 

 Distilled water 

 Formalin (40% formaldehyde) 



300 ml. 



13 gm. 

 150 ml. 



50 ml. 



DEL RIO - HORTEGA'S FIXATIVE 



Uranium nitrate 5% 

 Formalin (40% formaldehyde) 

 Distilled water 



75 ml. 

 25 ml. 

 150 ml. 



DREW'S FIXATIVE 



Sodium chloride 1% 



Cobalt nitrate 



Formalin (40% formaldehyde) 



400 ml. 

 10 gm. 

 100 ml. 



DURIG'S FIXATIVE 



Potassium dichromate 10% 

 Formalin (40% formaldehyde) 

 Distilled water 



150 ml. 

 50 ml. 

 350 ml. 



EMIG'S FIXATIVE 



Copper acetate 1% aqueous . . • • 75 Til- 

 Glacial acetic acid . . . . . . . . 7*5 ml. 



Formalin (40% formaldehyde) . . • • 75 rnl. 



Methyl alcohol, absolute 75 n^l- 



Note: The normal use of this fixative is in botany (for algae). 



GILSON'S ACETO ALCOHOL CHLOROFORM FIXATIVE 



Absolute alcohol 100 ml. 



Glacial acetic acid . . . . . . . • 100 ml. 



Chloroform 100 ml. 



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