SECTION THREE 



MANN'S FIXATIVE 



Osmic acid i% aqueous 

 Sodium chloride, pure 



100 ml. 

 075 gm. 



MAYER'S FIXATIVE 



Distilled water . . . . . . . . 250 ml. 



Nitric acid, cone. . . . . . . . . 6-25 ml. 



Picric acid . . . . . . . . • • 4 S^- 



Shake at intervals over a period of several hours; then filter. 



MULLER'S FLUID 



Potass, dichromate . . 

 Sodium sulphate 

 Distilled water 



NOVAK'S FIXATIVE 



2-5 gm. 

 I gm. 

 100 ml. 



Immediately before use add mercuric chloride, 16 gm. 



ORR'S FIXATIVE 



Osmic acid 2% 



Acetic acid i% aqueous 



80 ml. 

 20 ml. 



PICRIC NITRIC ACID 



Picric acid, aqueous, sat. 

 Nitric acid, cone. . . 



95 ml. 

 5 ml. 



PICRO-SUBLIMATE-ACETIC ACID (RATH'S FLUID) 



Picric acid, saturated, aqueous . . . . 50 ml. 



Mercuric chloride, hot saturated aqueous 50 ml. 

 Glacial acetic acid . . . . . . . . 40 ml. 



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