STAINING, PRACTICAL AND THEORETICAL 



HYDROGEN ION INDICATORS 

 ALIZARIN 



pH 4-0-6-8 and ii •0-13-0 



Alizarin . . . . . . . . . . 0-5 gm. 



Alcohol 70% . . . . . . . . 100 ml. 



ALIZARIN RED 



pH 4-0-6 -8 and 11-0-13-0 



Alizarin red . . ,. .. .. .. 0-5 gm. 



Distilled water . . . . . . . . 80 ml. 



Absolute alcohol . . . . . . . . 20 ml. 



ALIZARIN YELLOW G 



pH 10-1-12-1 



Alizarin yellow G .. .. .. .. o-i gm. 



Alcohol 50% 100 ml. 



ALIZARIN YELLOW GG 



pH 10-0-12-0 



Alizarin yellow GG .. .. .. o-i gm. 



Alcohol 50% 100 ml. 



BROMOCRESOL GREEN 



pH 3-6-5-2 



Bromocresol green . . . . . . . . 0-04 gm. 



N/iooNaOH 5-72 ml. 



Distilled water 74*28 ml. 



Absolute alcohol . . . . . . . . 20 ml. 



BROMOCRESOL PURPLE 



pH 5-2-6-8 



Bromocresol purple . . . . . . 0-04 gm. 



N/iooNaOH 7-4 ml. 



Distilled water . . . . . . . . 72-6 ml. 



Absolute alcohol . . . . . . . . 20 ml. 



BROMOPHENOL BLtJE 



pH 3-0-4-6 



Bromophenol blue . . . . . . 0-04 gm. 



540 



