SECTION THREE 



REAGENTS, SUNDRY 



ACETONE - XYLOL 



Acetone, absolute . . . . ■ . . . . loo ml. 



Xylol (water-free) 400 ml. 



This reagent clears tissues and sections from 90% alcohol. 



ALKALINE ALCOHOL 



Alcohol 90% 99"5 ml. 



Ammonium hydroxide (sp. gravity o-88) 0-5 ml. 



CARBOL XYLOL (Phenol Xylol) 



Xylol, anhydrous 300 ml. 



Phenol crystals 100 gm. 



Dissolve by shaking. 



EAU DE JAVELLE 



A. Bleaching powder . . . . • • 5° g'^- 

 Water 250 ml. 



Shake well ; then allow to stand for several hours in a closed bottle . 



B. Potassium carbonate . . . . . . 37*5 gm. 



Water 250 ml. 



Mix solution A with solution B; allow the mixture to stand for 

 several hours; then decant or filter through glass wool. 



FARRANTS' MEDIUM 



Gum acacia powder . . . . . . 150 gm. 



Water 150 ml. 



Warm the water to about 40*' C. ; sprinkle the gum on the surface of 

 the water a little at a time and stir in with a glass rod: then stir in: 

 Arsenious oxide . . . . . . . . 0-5 gm. 



Leave overnight; then stir well before adding: 



Glycerine 15° nil- 

 Stir well; filter through surgical lint, or decant after standing for 

 several days or weeks, if necessary. 



GLYCERINE ALBUMEN (Mayer) 



*Egg albumen . . . . . . . . 100 ml. 



Glycerine . . . . . . • . • • 100 ml. 



Sodium salicylate . . . . . . . . 2 gm. 



Shake well; allow to stand in a closed glass vessel for several hours; 

 then filter if necessary. 



* Four or five fresh eggs are required to produce 100 ml. of albumen. 



545 



