STAINING, PRACTICAL AND THEORETICAL 



GLYCERINE JELLY 



Gelatine powder . . . . . . . . lOO gm. 



Water . . . . . . . . . . 650 ml. 



Warm the water, in a 2-litre beaker or an enamel bowl, to about 

 40-50° C; then sprinkle the gelatine on the surface of the water a little 

 at a time, stirring in each lot with a glass rod ; then add and stir in : 

 Glycerine . . . . . . . . . . 700 gm. 



Pour the mixture into screw cap storage jars, and add a crystal of 

 thymol to each. 



Keep the jars tightly closed. 



LACTOPHENOL (Amann) 



Lactic acid . . . . . . . . . . 16 ml. 



Glycerine 

 Distilled water 

 Phenol, cryst. 



31ml. 



. . 20 ml. 

 . . 20 gm. 



Warm on a water bath until the phenol has gone into solution; then 

 store in a dark glass bottle. 



SCOTT'S TAP WATER SUBSTITUTE 



Sodium bicarbonate 0-7% aqueous . . i volume 



Magnesium sulphate, cryst., 4% aqueous i volume 



STAINS AND REAGENTS 



ACETIC THIONIN 



Thionin (Ehrlich) . . . . . . . . c-i gm. 



Distilled water . . . . . . . . 120 ml. 



Glacial acetic acid . . . . . . . . 6 ml. 



ACETO CARMINE (SCHNEIDER) 



Carmine . . . . . . . . . . 0*4 gm. 



Distilled water . . . . . . • • 55 nil- 



Heat to boiling, then add: 



Glacial acetic acid . . . . . . . . 45 ml. 



Raise to boiling point again; then cool and filter. 



ACID FUCHSIN (ALTMANN) 



Aniline water . . . . . . . . 100 ml. 



Acid fuchsin . . . . . . . . 20 gm. 



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