STAINING, PRACTICAL AND THEORETICAL 



To prepare the staining solution: 

 Aldehyde-fuchsin, prepared as 

 above .... •• •• 0-25 gm. 



Absolute alcohol . . • • 35 i^^- 



Distilled water .. •• 15 "il- 



Note: This solution will keep for at least sk months. 



D. Halmi's mixture (as page 349, 



solution G) 



E. Alcohol, 96% 25 ml. 



Acetic acid, glacial . . • • i drop 



Technique: 



1. Attach paraffin sections to slides, and dewax with xylol. 



2. Hydrate through the usual graded alcohols. 



3. Oxidize in solution A (Gomori's fluid), for one minute. 



4. Rinse in solution B until all permanganate stain has been 

 removed. 



5. Wash in distilled water. 



6. Wash with 30% alcohol. 



7. Wash with 70% alcohol. 



8. Immerse in aldehyde-fuchsin stain (solution C), for two to 

 ten minutes, in a closed dish or tube. 



9. Immediately wipe the back of the slide and rinse in 95% 

 alcohol. 



10. Immerse the slide in a second lot of 95% alcohol for two 

 to five minutes, until no more aldehyde-fuchsin comes out of 

 the section. 



11. Wash in 70% alcohol. 



12. Rinse in 30% alcohol. 



13. Rinse in water. 



14. Counterstain in Halmi's mixture for twenty to thirty 

 seconds. 



15. Wipe the back of the slide, then diiferentiate in solution E 

 until no more colour comes out of the section. 



16. Rinse in 95% alcohol. 



112 



