SECTION TWO 



17. Dehydrate with absolute alcohol. 



18. Mount in Michrome mountant or: 



19. Treat with a second lot of absolute alcohol. 



20. Clear in xylol. 



21. Mount in D.P.X., Clearmount, Emexel or Michrome 

 mountant. 



Notes: 



{a) The method incorporates some of Gabe's (Gabe, 1953) 

 modifications of Gomori's aldehyde-fuchsin (Gomori, 1950) 

 together with the counterstain of Halmi (1952), and the authors 

 state that it has been used in their laboratory for two years with 

 complete success. 



{b) The authors state that it was found unnecessary to acidify 

 the aldehyde-fuchsin staining solution with acetic as in Gabe's 

 modification of Gomori, since in the staining of neurosecretory 

 material no difference could be seen between sections stained with 

 and without the acid, 



{c) For more detailed information, reference should be made to 

 the original paper. 



Reference: Cameron, M.D. & Steele, J. E. (1959). 



ALDEHYDE FUCHSIN-LUXOL FAST YELLOW - 

 PONTACYL BLUE BLACK 



For the differential staining of elastic fibres, collagen 



fibres and mucin 



113 



