STAINING, PRACTICAL AND THEORETICAL 



room temperature. Next morning shake with about 

 I gm. of decolorizing charcoal before filtering. 

 Store in a refrigerator. 



B. Pontacyl blue black SX 

 Water 



C. Potassium dichromate 

 Distilled w^ater 



D. Solution B 

 Solution C 



E. Fast yellow TN 

 Alcohol, 95% 



I gm. 



100 ml. 



2gm. 



100 ml. 



30 ml. 



10 ml. 



2gm. 



100 ml. 



Technique: 



1. Fix pieces of tissue in Bouin or Zenker or formalin. 



2. Embed in paraffin wax as usual. 



3. Fix sections to slides and remove paraffin wax with xylol. 



4. Wash in absolute alcohol. 



5. Wash in 90% alcohol. 



6. Wash in 70% alcohol. 



7. Treat for the removal of mercurial deposits if a fixative 

 containing mercury has been used. 



8. Rinse in 70% alcohol. 



9. Immerse in Gomori's aldehyde fuchsin (solution A) for 



thirty minutes. 



10. Rinse well in 70% alcohol. 



11. Rinse in distilled water. 



12. Immerse in the pontacyl blue black SX-bichromate (solu- 

 tion D) for fifteen minutes. 



13. Rinse in tap water. 



14. Differentiate by dipping the slide into 70% alcohol untd 

 no more blue colour runs out of the section. 



15. Immerse in the fast yellow TN (solution E) for five minutes. 



16. Rinse in 95% alcohol. 



17. Dehydrate in absolute alcohol. 



18. Clear in xylol. 



19. Mount in Clearmount or D.P.X. or Emexel. 



Results : 



Elastic fibres: deep red. Collagen: brilliant yellow. Mucin of 

 goblet cells: reddish purple. Cytoplasmic granules in cells of 



114 



