STAINING, PRACTICAL AND THEORETICAL 



ALDEHYDE FUCHSIN - HAEMATOXYLIN LIGHT GREEN - 

 ORANGE G - CHROMOTROPE 2R 



For the differentiation of two types of Basophils in the 

 Adenohypophysis of the rat and the mouse 



Solutions required: 



A. Bouin's fixative with the acetic acid replaced 

 by 0*5% trichloracetic acid. 



B. Lugol's Iodine 



C. Sodium thiosulphate 5% aqueous 



D. Aldehyde-fuchsin 

 Basic fuchsin 

 Absolute alcohol 

 Distilled water 

 Paraldehyde . . 

 Hydrochloric acid, cone. 



0-5 gm. 

 60 ml. 

 40 ml. 

 I ml. 

 I -5 ml. 



Note: The solution turns purple in 24 

 hours, and is ripe and ready for use after being 

 kept at 20° C. for three days or in two days if 

 kept at about 37° C. The stain deteriorates 

 after four or five days. 



E. Ehrlich Haematoxylin 



F. Ethyl alcohol 70% 99 '5 ^1. 



Hydrochloric acid, cone 0-5 ml. 



G. Lithium carbonate, saturated aqueous 



H. Orange G 2% aqueous . . . . 50 ml. 



Light Green SF 1% aqueous . . 20 ml. 



Distilled water 30 ml. 



Chromotrope 2R 0-5 gm. 



Phosphotungstic acid . . . . 0-5 gm. 



Glacial acetic acid i ml. 



Dissolve the phosphotungstic acid in the 

 distilled water, then add and dissolve the 

 chromotrope 2R followed by the acetic acid, 



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