STAINING, PRACTICAL AND THEORETICAL 



19. Counterstain in the light green-orange-chromotrope (solu- 

 tion H) for 45 seconds. 



20. Rinse quickly with 0-2% acetic acid. 



21. Rinse in 95% alcohol. 



22. Immerse for two minutes in each of two changes of absolute 

 alcohol. 



23. Blot slides carefully. 



24. Immerse in xylol for two minutes. 



25. Immerse in another lot of xylol for five minutes. 



26. Mount in D.P.X. or Clearmount. 



Results : 



Granulation of beta cells, selectively stained dark purple with 

 the aldehyde-fuchsin. The cells of the pas intermedia and the 

 Herring bodies of the neutral lobe should have little or no affinity 

 for aldehyde-fuchsin. The delta cells are stained green, and the 

 acidophilic granules varying shades of orange. Nuclear chromatin, 

 purplish brown to reddish brown. Nucleoli are tinged bright 

 red, by the chromotrope 2R. The non-granular cytoplasm, 

 greyish green or unstained. Coagulated contents of the cyto- 

 plasmic vacuoles, orange. 



Reference: Halmi, Nicholas S. (1952). 



ALDEHYDE FUCHSIN - PERIODIC ACID - SCHIFF' 



COMBINED 



For staining the pituitary 



Solutions required: 



A. Mercuric chloride . . • • 25 gm. 

 Distilled water 500 ml. 



B. Solution A 100 ml. 



Chrome alum . . . . • • 5 g^^- 



Formahn (formaldehyde 40%) . . 5 ml. 



Note: Solution B should be made up freshly in 

 quantities sufficient for the day's use. 



118 



