SECTION TWO 



10. Stain in the well-ripened aldehyde thionin (solution E) for 

 ten minutes. 



1 1 . Rinse in water. 



12. Immerse in 70% alcohol for a few seconds. 



13. Immerse in 95% alcohol for a few seconds. 



14. Stain in the luxol fast blue (solution F) for thirty minutes 

 maintaining the temperature at 57° C for the whole of this 

 period. 



15. Rinse in 95% alcohol. 



16. Rinse in 70% alcohol. 



17. Rinse in water. 



18. Differentiate in the lithium carbonate (solution G). 



19. Immerse for a few seconds in each of four changes of 70% 

 alcohol. 



20. Wash in water. 



21. Immerse in the periodic acid solution for ten minutes. 



22. Rinse in water. 



23. Immerse in Schiff's reagent for thirty minutes. 



24. Wash in tap water for ten minutes. 



25. Dehydrate through the usual graded alcohols. 



26. Clear in xylol and mount in Canada balsam in xylol, 

 D.P.X., Cristalite or Clearmount. 



Results: 



Thyrotrophs are stained intense blue-black by the aldehyde 

 thionin, the granules of the thyroidectomy being particularly 

 well shown. Gonadotrophs are easily distinguishable, stained the 

 clear red of the PAS technique, as are also the basement mem- 

 branes of the blood vessels. Acidiphils are stained a clear intense 

 blue-green. Chromophobes are unstained or very lightly stained 

 by the luxol fast blue, depending upon the degree of differentia- 

 tion which has been carried out. 



Notes: 



(a) The authors state that the whole pituitary presents a 

 brilliant appearance of clarity, enabling cell counts to be per- 

 formed easily by individuals unversed in the morphology of 

 pituitary cells, purely by reference to their colour. The secretions 

 of the pars intermedia and pars posterior are also brilliantly 

 stained. It is also stated that although other stains (e.g. orange G) 



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