STAINING, PRACTICAL AND THEORETICAL 



Note: 



Lillie designated this procedure as the " Allochrome " method 

 denoting the change of colour of collagen, and some related 

 substances, from red to blue, during the process (allochrome = of 

 different or changing colour) which is a sequence of Schiff's 

 leucofuchsin, picro aniline blue. 



Reference: Lillie, R. D. (195 1). 



AMMONIACAL SILVER CARBONATE 



For vascular reticulum, tumour cells, connective tissues 

 around tumour, in abnormal brain tissue 



Solutions required: 



A. Pyridin, pure . . . . . . 2 volumes 



Glycerin, pure . . . . i volume 



B. Ammoniacal Silver Carbonate 



Ammonia solution is added drop by drop to 10 ml. 

 silver nitrate 10-2% until the precipitate formed is 

 almost redissolved, leaving a slightly opalescent solution 

 to which is then added 10 ml. sodium carbonate 

 3-1% solution and sufficient distilled water to make 

 the volume up to 100 ml. 



C. Reducing solution 



Sodium carbonate anhydrous i gm. 



Formalin . . . . . . i ml. 



Distilled water . . . . . . 103 ml. 



D. Brown gold chloride o-2% aqueous. 



E. Intensifying solution 



Oxalic acid 2% aqueous . . 100 ml. 



Formalin . . . . . . i ml. 



F. Sodium hyposulphite 10% aqueous. 



Technique: 



The material is fixed in 10% formalin or in Bouin and embedded 

 in paraffin wax. 



I. Bring sections down to distilled water and immerse in Solu- 

 tion A for twenty-four hours. 



132 



