SECTION TWO 



2. Wash with 95% alcohol, then with distilled water. 



3. Immerse in Solution B for two and a half hours at 40° C. 



4. Wash with distilled water; then reduce in Solution C for 

 five minutes, afterwards washing in tap water. 



5. Tone for five minutes in Solution D at 30° C. ; then wash in 

 tap water. 



6. Intensify by immersing in Solution E for five minutes ; then 

 rinse in tap water. 



N.B. — The above stages must be carried out in the darkroom. 



7. Fix in Solution F. 



(Note: Fixation should be completed in fifteen to twenty 

 minutes.) 



8. Wash in tap water; dehydrate; clear and mount. 



Results: 



Tumour cells: reddish to greyish violet. Vascular reticulum: 

 black. 



Reference: Conn, H. J. & Darrow, Mary A. (1947), 



Important.— The tissues must not be allowed to come 

 into contact with mercuric chloride, as even a trace will 

 ruin the preparation. 



ANILINE BLUE - FUCHSIN ACID 

 For elementary bodies in animal sections 



B. 



133 



