STAINING, PRACTICAL AND THEORETICAL 



C. Acid fuchsin i% aqueous 



Oxalic acid 3% 



100 ml. 

 2 ml. 



Technique: 



1. Pieces of tissue are fixed for twenty-four hours in Solution A; 

 washed, dehydrated, cleared and embedded in paraffin wax as 

 usual. 



2. Sections, not thicker than $^1, are fixed to slides, de-waxed and 

 taken through descending grades of alcohol down to water as 

 usual. 



3. Stain for one half to one hour in the aniline blue (Solution B) 

 in a stoppered staining jar. 



4. Rinse well with distilled water. 



5. Drain and blot carefully to remove excess water. 



6. Rinse in absolute alcohol. 



7. Stain for twenty minutes in the acid fuchsin (Solution C). 



8. Pour off; drain and blot carefully to remove excess liquid. 



9. Dehydrate with absolute alcohol; clear in xylol and mount 

 in cristalite or in Canada balsam. 



Results: 



Elementary bodies in cells associated with the following viruses 

 are stained scarlet: borna, zoster, rabies and pseudo rabies. 



Reference: Nicolau, S. & Kopciowska, L. (i937)- 



ANILINE BLUE - EOSIN B 



A simple and rapid technique for spermatozoa, which is 

 particularly suitable for dog and human semen 



B. 



Solutions required: 



Ether 



Absolute alcohol 



Aniline blue aqueous 

 Eosin, B 



Phenol 1% aqueous 

 Distilled water 



I volume 



1 volume 



2 gm. 

 I gm. 

 20 ml. 

 60 ml. 



134 



