STAINING, PRACTICAL AND THEORETICAL 



ANILINE CRYSTAL VIOLET - GRAM'S IODINE 

 For epithelial fibres 



Solutions required: 



A. Aniline crystal violet. 



B. Gram's iodine. 



C. Aniline xylol. 



Technique: 



1. Material should be fixed in absolute alcohol and embedded 

 in paraffin wax. 



2. Sections, not more than ^/li thick, are fixed to slides and 

 brought down to distilled water in the usual manner. 



3. Stain for ten to fifteen minutes in aniline crystal violet. 



4. Wash well in running water. 



5. Stain with Gram's iodine for ten to thirty seconds. 



6. Wash in water; drain; then blot carefully but thoroughly to 

 remove water. 



7. Differentiate with aniline xylol, controlling at frequent inter- 

 vals by examination under the microscope. 



8. Wash well with xylol ; mount in balsam or D.P.X. 



Results: 



Epithelial fibres are stained blue. 



ANILINE CRYSTAL VIOLET - LITHIUM CARMINE - 



IODINE 



For fibrin and for Gram-positive organisms in animal 



tissues 



136 



