STAINING, PRACTICAL AND THEORETICAL 



6. Wash quickly in distilled water; then differentiate in 95% 

 alcohol followed by absolute alcohol. 



7. Clear and mount. 



Results: 



Collagen stained deep blue; reticulum, deep blue; chromatin, 

 red; muscle tissue, reddish to orange; erythrocytes, red; neur- 

 oglia, reddish; mucin, blue. 



Reference: Heidenhain, M. (19 15). 



AZO CARMINE - MALLORY STAIN 

 For Islets of Langerhans 



Solutions required: 



A. Azocarmine, B, aqueous o-i% . . 100 ml. 

 Boil for about 5 minutes; then cool and add 

 2 ml. glacial acetic acid. Then warm to 60 °C. 

 and filter at that temperature. 



B. 90% alcohol 99 ml. 

 Aniline Oil i ml. 



C. Iron alum 5% aqueous. 



Aniline Blue - Orange G (Mallory) : 



D. AniUne blue, aqueous . . . . 0-5 gm. 



Orange G. . . . . . . . . 2 gm. 



Distilled water . . . . . . 100 ml. 



E. Solution D . . 

 Distilled water 



I volume 

 2 to 3 volumes 



Technique: 



1. Fix thin slices of pancreas in Bouin for eight to ten hours. 



2. Wash in distilled water, dehydrate, clear and embed in 

 paraffin wax. 



3. Cut section 4/^ in thickness. 



140 



