SECTION TWO 



4. Fix section to slides; dewax and take down through the 

 usual grades of alcohol to distilled water. 



5. Stain in solution A for about forty-five to sixty minutes at 

 56° C. 



6. Rinse quickly in distilled water and blot very carefully. 



7. Destain in solution B until acinous tissue is almost colourless 

 and B cells show red against pink background of A cells. 



8. Rinse briefly with distilled water and treat with 5% iron 

 alum solution for 5 minutes or more. 



9. Rinse again and stain two to twenty minutes in solution E 

 until the collagenic tissue appears deep blue under the microscope. 



ID. Rinse and blot carefully. 



1 1 . Differentiate and dehydrate in absolute alcohol. 



12. Clear in xylol and mount. 



Results: 



Cytoplasm of A cells: rich yellow orange; of B cells: bright 

 red ; and of D cells : sky blue. 



Note: It is stated that it can be demonstrated that there is no gradation 

 between A and B cells by first staining with Neutral Gentian (Bensley) 

 decolorizing, then restaining by the above technique. 



References : 



Gomori, G. (1939). 

 Cowdry, E. V. (1952). 



AZOCARMINE - HAEMATOXYLIN - ACID GREEN - 



ORANGE G 



For difTerential cell analysis of the rat anterior hypophysis 



Solutions required: 



A. Zenker - Formol 



Potassium dichromate . . ■ • 25 gm. 



Mercuric chloride . . . . . . 50 gm. 



Ringer's solution (i.e. 0-9% saline) . . i litre. 



Add I ml. neutral formaldehyde 40% solution per 10 ml, of the 

 above solution immediately before using. 



141 



