SECTION TWO 



(b) It should be mentioned that alloxan is considerably less 

 expensive than ninhydrin. 



References : 



Shanklin, W. M. & Issidorides, M. (i960). 



Lillie, R. D. (1954). 



Yasuma, A. & Ichikawa, T. (1953). 



BAUER - FEULGEN STAIN 

 For Glycogen, etc. 



Solutions required: 



A. Chromium trioxide 4 % aqueous 



B. Feulgen's fuchsin (SchifF's reagent) 



C. Sodium metabisulphite 10-4% 



aqueous . , . . . . • • 5 nil. 



Tap water . . . , . . • • 95 ml. 



Technique: 



1. Fix fresh material immediately in alcohol and embed in 

 paraffin wax. 



2. Dewax sections; pass through the usual descending grades 

 of alcohol to distilled water. 



3. Immerse in solution A for one hour. 



4. Wash in running water for five to ten minutes. 



5. Immerse in the Feulgen fuchsin for ten to twenty minutes. 



6. Agitate the slides gently for about two minutes in each of 

 three changes of solution C. 



7. Wash in running water for ten minutes. 



8. Stain nuclei, if desired, in haemalum, for two to five minutes. 



9. Dehydrate as usual; clear in xylol and mount. 



Results: 



Glycogen: intense reddish-violet. Nuclei: pale mauve to navy 

 blue (if haemalum is used). 



Reference: Bauer, H. (1933). 



