SECTION TWO 



time after neutral formalin fixation, red corpuscles and haemo- 

 globin may sometimes give the Prussian blue reaction of Perls. 



References : 



Lillie, R. D. (1948). 

 Perls, M. (1867). 

 Pickworth, F. A. (1934). 



BIEBRICH SCARLET - ETHYL VIOLET - HAEMATOXYLIN 



A modification of Bowie's stain for pepsinogen granules of 

 the body chief cells in the gastric glands 



N.B. — This solution must be freshly pre- 

 pared each time it is required for use. 



D. Clove Oil . . . . . . . . I volume 



Toluol . . . . . . . . I volume 



Technique: 



1 . Fix in Regaud's fluid for five days in a dark phial placed in a 

 larger amber bottle which should be wrapped round with a thick 

 cloth and kept in a dark room. Change the fixative daily. 



2. Dehydrate with normal propyl alcohol {see page 514). 



3. Transfer to paraffin wax which should be changed three times 

 before the block is finally cast. 



4. Cut sections 5 to 6ja in thickness and fix to slides as usual. 



5. Remove wax with two changes of xylol. 



6. Pass through absolute alcohol and the usual descending grades 

 of alcohols down to distilled water. 



7. Stain in the Haematoxylin solution for i minute. 



H7 



