SECTION TWO 



4. Dehydrate with 95% and absolute alcohol; then clear in 

 xylol, and mount. 



Results: 



Cartilage: dark brown. Mucin: light brown. Nuclei of all 

 cells: green. 



Notes: 



The authors state that the method is essentially the same as 

 List (1885). 



Reference: Conn, H. J. & Darrow, Mary A. (1947), Section A: "Animal 

 Histology," p. 5. 



BIONDI - EHRLICH - HEIDENHAIN STAIN 

 For chromatin, nucleoli, mucin, etc. 



Solution required: 



Biondi - Ehrhch - Heidenhain 0-9 gm. 

 Distilled water . . . . . . 100 ml. 



Dissolve by warming and stirring in a beaker. 

 When cool add : 



Chloroform . . . . . . 0-25 ml. 



Technique: 



1 . Fix tissues in saturated aqueous mercuric chloride and embed 

 in paraffin wax in the usual manner. 



2. Fix sections to slides; de-wax with xylol and pass through 

 absolute alcohol followed by 90% and 70% alcohol. 



3. Treat for the removal of mercuric precipitate by the standard 

 technique. 



4. Immerse in the staining solution from six to twenty-four 

 hours. 



5. Rinse directly with 95% alcohol. 



6. Dehydrate with absolute alcohol. 



7. Clear in xylol and mount. 



F 149 



