STAINING, PRACTICAL AND THEORETICAL 



Results: 



Chromatin is stained bluish green, while nucleoli are red; 

 mucin is stained green; erythrocytes, orange. Cytoplasm and 

 connective tissue elements are in varying shades of red. 



References : 



Ehrlich, P. & Lazarus, A. (1898). 

 Heidenhain, M. (1892). 

 Pfluger Arch. (1888). 



BEST'S CARMINE 

 For glycogen 



Solutions required: 



A. Ehrlich haematoxylin. 



B. Best's carmine stock solution . . 10 ml. 

 Methyl alcohol, pure . . . . 15 rnl. 

 Strong ammonia solution . . 10 ml. 



Note: This solution should not be prepared until 

 it is required for immediate use. 



C. Celloidin 1% in equal volumes of 



absolute alcohol and ether. 



D. Absolute (ethyl) alcohol . . . . 80 ml. 

 Absolute (methyl) alcohol . . 40 ml. 

 Distilled water 100 ml. 



Technique: 



1. Tissues are fixed in Bouin Fluid and embedded in Celloidin 

 or in paraffin wax. If Celloidin sections are employed proceed as 

 from stage 5 (below). If paraffin sections are used the procedure 

 is as follows: 



2. Float sections on the slide with 70% alcohol; flatten out; 

 then remove excess alcohol with filter paper and blot carefully but 

 thoroughly. 



3. Remove paraffin wax with xylol in the usual manner. 



4. Wash with absolute alcohol as usual. 



150 



