STAINING, PRACTICAL AND THEORETICAL 



Technique: 



1. Tissue should be fixed for one to three weeks in io% for- 

 malin and frozen sections, 200 to 300^f should be employed. 



2. Immerse in several changes of distilled water for a total 

 period of two hours. 



3. Immerse in Solution C for half an hour at 37° C, agitating 

 at frequent intervals. 



4. Wash in several changes of distilled water. 



5. Immerse in Solution D for half an hour at 37° C. agitating 

 frequently. 



6. Wash in distilled water. 



7. Dehydrate by passing through ascending strengths of alcohol, 

 beginning with 70%, in the usual manner. 



8. Clear in xylol and mount. 



Results: 



Blood vessels are stained black agamst a background which is 

 almost colourless. 



Note: 



Cowdry (1952) states that this is a simplified Lephen-Pick- 

 worth peroxidase method, due to Campbell and Alexander. 



References : 



Mallory, F. B. (1938), p. 257. 

 Cowdry, E. V. (1952). P- 56. 



BRILLIANT CRESYL BLUE 

 For reticulated cells and platelets 



Solutions required: 



A. Brilliant Cresyl Blue 0-3% absolute 



ethyl alcohol. 



B. Leishman stain or Wright's stain. 



Technique : 



I. Place a drop of 0-3% BrilHant Cresyl Blue stain in absolute 

 alcohol on a sHde and allow it to dry. 



152 



