STAINING, PRACTICAL AND THEORETICAL 



CARBOL ANILINE FUCHSIN 

 For Negri bodies 



Technique: 



1 . Fix tissues in Zenker's Fluid for twenty-four hours ; wash in 

 running water for two or three hours ; dehydrate ; clear ; embed 

 in paraffin wax in the usual manner. 



2. Sections, 4 to 5^ in thickness, are stained from ten to thirty 

 minutes in Solution A; then washed with distilled water. 



3. Stain with Methylene Blue (Loeffler) for fifteen to sixty 

 seconds ; then wash with water. 



4. Dehydrate and differentiate for a few seconds in absolute 

 alcohol; then clear in xylol and mount. 



Results: 



Negri bodies are stained crimson against a blue background. 



Note: The method is stated to be excellent for Borrel bodies 

 also. 



Reference: Goodpasture, E. W. (1925). 



CARBOL CRYSTAL VIOLET 



For fibrin network in blood smears 



Solution required: 

 Carbol Crystal Violet 

 Technique : 



Note: To retain the normal arrangement of the filaments in the fibrin 

 network, the slides must be slowly and carefully placed in, and taken 

 out of the reagents. 



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