STAINING, PRACTICAL AND THEORETICAL 



Techiique: 



1. Fix pieces of tissue in io% neutral formalin. 



2. Embed in paraffin wax or celloidin or cut frozen sections. 



3. Take sections down to distilled water in the usual way. 



4. Immerse in reagent A for three to four hours at 60° C. 



5. Wash well in running water. 



6. Rinse in distilled water. 



7. Differentiate in solution B, controlling under the microscope 

 until the red cells are faint pink in colour. 



8. Wash well in distilled water. 



9. Counterstain in the haematoxylin for five minutes. 



10. Wash and " blue " in tap water or in lithium carbonate 

 solution. 



1 1 . Wash in distilled water. 



12. Dehydrate through the usual graded alcohols. 



13. Clear in xylol. 



14. Mount in Clearmount or in D.P.X. or Emexel. 



Results: 



Acid fast lipofuscins are stained a brilliant red, while lipo- 

 proteins are pink, and nuclei are blue or dark blue. 



References : 



Gomori, G. (1952). 



Lillie, R. D. (1948). 



Pearse, A. G. Everson (1953). 



CARBOL FUCHSIN (Ziehl Neelsen) 

 For Nissl Bodies 



Solutions required: 



A. Carbol fuchsin (Ziehl Neelsen) . . 10 ml. 

 Acetic acid 0-5% . . . . 10 ml. 



B. Acetic acid I % .. .. .. 10 ml. 



Formalin .. .. .. .. o-i ml. 



Technique: 



I. Small pieces of tissue are fixed in 10% formalin or in 95% 

 alcohol or in formol-saline for at least twenty-four hours, and 



156 



