STAINING, PRACTICAL AND THEORETICAL 



Shake the borax and carmine with the water : then boil gently for 

 thirty minutes ; allow to cool ; make up to the original volume with 

 distilled water; then add the loo ml. 70% alcohol. Mix well and allow 

 to stand several days before filtering. 



BORAX CARMINE (MAYER) 



Borax . . . . . . . . . . i gm. 



Carmine . . , . . . . . . . i gm. 



Distilled water , . . . . . . . 50 ml. 



Absolute alcohol . . . . . . . . 50 ml. 



Shake the borax and carmine with the water: then boil gently for 

 thirty minutes; cool; make the volume up to 50 ml. with distilled 

 water; add the alcohol; then filter. 



BORREL'S BLUE 



Borrel's blue powder (Michrome No. 376) 2 gm. 

 Distilled water . . . . . . . . 50 ml. 



Boil for five minutes: then cool and filter and make the volume up 

 to 50 ml. with distilled water. 



BRAZILIN (HICKSON) 



A. Iron alum 10% aqueous . . . . 10 ml. 



Distilled water . . . . . . 20 ml. 



Absolute alcohol . . . . . . 70 ml. 



B. Brazilin 0-5% in 70% alcohol 



CARBOL FUCHSIN (ZIEHL NEELSEN) 



Basic fuchsin 10% in absolute alcohol . . 10 ml. 

 Phenol 5 % aqueous .. .. .. 100 ml. 



Mix well and allow to stand overnight before filtering. 



CARBOL GENTIAN VIOLET 



Gentian violet 10% in absolute alcohol . . 10 ml. 

 Phenol 2% aqueous . . . . . . 100 ml. 



Alcohol 95 % . . . . . . . . 20 ml. 



Mix well ; then filter. 



CARBOL METHYLENE BLUE 



Phenol 5% aqueous . . . . . . 100 ml. 



Methylene blue . . . . . . . . i gm. 



Absolute alcohol . . . . . . . . 10 gm. 



