SECTION THREE 



CARBOL THIONIN 



Thionin (Ehrlich) 0-125 gm. 



Phenol crystals ^ g"^- 



Alcohol 10% 100 ml. 



■ Heat to dissolve, or triturate in a mortar; filter when dissolved. 

 The solution deteriorates after a few weeks. 



CARBOL TOLUIDINE BLUE 



Toluidine blue i g"^- 



Phenol (cryst.) S'O gm- 



Distilled water 100 ml. 



CARMALUM (MAYER) 



Carminic acid . . • • • • . • i gni. 



Potash alum 5% 200 ml. 



Boil for half an hour. Cool and filter. Add a crystal of thymol or 

 a few drops of chloroform, to the stock bottle, as preservative. 



CARMINE (DE GROOT) 



Iron alum 10% aqueous 0-5 ml. 



Potash alum 10% aqueous . . • • 25 ml. 



Carminic acid . . • • • • . . i gm. 



Distilled water 74"5 ml. 



Hydrochloric acid, cone. . . • • . . i drop 



Boil the carminic acid with the water; then shake in the other in- 

 gredients and filter. 



CARMINE (Meriwether) 



Potassium carbonate 10% aqueous . . 19 ml. 



Potassium chloride 10% aqueous . . 62-5 ml. 



Carmine 2-5 gm. 



Distilled water 18-5 ml. 



Boil for two minutes; cool and filter; then add: 



Ammonium hydroxide (sp. gravity o-88) 25 ml. 



CHROME HAEMATOXYLIN (GOMORI) 



Haematoxylin I % aqueous .. .. 50 ml. 



Chrome alum 10% aqueous . . . . 15 ml. 



Potassium dichromate 10% aqueous . . i ml. 



Sulphuric acid, cone o-i ml. 



